Tris(hydroxymethyl)aminomethane acetate salt

Tris HCl, abbreviated as Tris HCl, is a hydrochloride salt of trihydroxymethyl aminomethane. Tris HCl buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important applications. Tris HCl was used for protein crystal growth under different pH conditions. The low ionic strength characteristic of Tris HCL buffer can be used for the formation of intermediate fibers in the lamin of C. elegans. Tris HCl is also one of the main components of protein electrophoresis buffer. In addition, Tris HCL is also used as an intermediate in the preparation of surfactants, vulcanization accelerators, and some drugs. Tris HCl is also used as a titration standard. Preparation of buffer solution: Tris HCl buffer solution with a concentration of 6.057g/L and pH=8.8 was prepared by adjusting the pH value of HCl aqueous solution with a concentration of 0.1mol/Chemicalbook L using Tris HCl hydrochloride as the solute and deionized water as the solvent. Advantage 1. Appearance: Trimethylaminomethane acetate appears as a white crystalline powder and is soluble in organic solvents such as water. The application of trihydroxymethylaminomethane acetate can be used to prepare an electrolyte for lithium batteries. The main raw materials of the electrolyte for lithium batteries are 5-10 parts by weight of dimethyl sulfoxide, 8-12 parts of hydroxyurea, 25-30 parts of cytarabine hydrochloride, 6-8 parts of polyvinylpyrrolidone, 2-6 parts of potassium bis trifluoromethanesulfonamide, 1-3 parts of lithium hexafluorophosphate, 1-3 parts of 2,4-dichlorophenylhydrazine hydrochloride, 1-3 parts of trihydroxymethylaminomethane acetate, 1-3 parts of (4-aminophenyl) methylsulfonyl fluoride hydrochloride, and 0.5-1.2 parts of N, N-dimethyl-p-phenylenediamine oxalate. During the battery charging and discharging process, the electrolyte prepared by the present invention suppresses the capacity decay of the battery at low temperatures and can reduce the amount of gas generated by the electrolyte under high temperature conditions, effectively preventing battery expansion and improving the high-temperature performance of the battery. Purpose TAE electrophoresis buffer is the most commonly used buffer for DNA agarose gel electrophoresis, and also used for non denatured RNA agarose gel electrophoresis. Double stranded DNA tends to move faster in TAE than in other buffer solutions, but it may also be unable to swim during prolonged electrophoresis due to buffer ion depletion. During long-term electrophoresis, buffer circulation or buffer replacement can compensate for lower buffering capacity. Dilute and concentrate TAE buffer to obtain 1mMTAE buffer, containing 40mM Tris acetate and 1mM EDTA, with a pH of 8.3. 1mMTAE buffer can be used not only in agarose gel, but also as electrophoresis buffer. To achieve maximum resolution, it is recommended to apply an external voltage below 5V/cm (distance between unit electrodes). This product is the most sensitive buffer for detecting firefly luciferase ATP; Glutamate binding detection.