sequencing grade carboxypeptidase B

Sequencing Grade Carboxypeptidase B

Catalog Number:SRCPB01

CAS:9025-24-5
EC:3.4.17.2

Storage temperature: 2-8℃

Source: Sequencing grade recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from  the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.

2. MAIN FEATURES

3.RECOMMEND USAGE 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

Sequence grade recombinant protein: high specificity,good stability.

Animal origin free: Sequencing grade carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.PRODUCT USAGE

1) Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine.

2) detection of antibody basic peak.

7.RELATED PRODUCTS

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase