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Category:Bio Products > Reagent > Enzyme
Product Name:recombinant human trypsin
Price(USD):0.00
Company:Shanghai Yaxin Biotechnology Co., Ltd.
Factory Location: Shanghai,China
Main Sales Markets: North America,Central/South America,Western Europe,Eastern Europe,Australasia,Asia,Middle East,Africa
Sample Provided: no
Recombinant Human Trypsin
Catalog Number:RHT03
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature: 2-8℃
Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
Source |
Recombinant E. coli |
Appearance |
White or off white powder |
Specific activity |
≥2500 USP units/mg pro. |
Protein Content |
≥ 90% |
Purity(HPLC) |
≥ 95% |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
6.PRODUCT USAGE
Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant trypsin
Recombinant enterokinase
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