Basic Info
  • Factory Location: Shanghai,China

    Main Sales Markets: North America,Central/South America,Western Europe,Eastern Europe,Australasia,Asia,Middle East,Africa

  • Monthly Production Capacity: 5kg

    Contract Manufacturing: CRO,CMO

  • Delivery Lead Time: Within a week

    Sample Provided: no

    Payment Terms: Payment before Delivery

    Recombinant Aprotinin

    Catalog Number: RTI16

    CAS: 9087-70-1

    Synonyms: Trypsin Inhibitor

    Source: Recombinant aprotinin is a genetically engineered protein expressed in E.coli

    Storage temperature: 2-8℃

    Protein Sequence:The amino acid sequence of recombinant aprotinin is identical to bovine lung derived aprotinin

     

    1.DESCRIPTION

    Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. Effective concentration is equimolar with protease. YaxinBio recombinant aprotinin is a genetically engineered protein expressed in E.coli and purified by multi-column.The amino acid sequence of recombinant trypsin is identical to bovine lung derived aprotinin,with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes.

     

    2.MAIN FEATURES

    Source

    Recombinant E. coli

    Appearance

    White or off white, or yellowish powder

    Solubility (5mg/ml in 0.9%NaCl)

    Clear to very slight hazy

    Protein Content [E1% A280 nm = 8.4]

    ≥60%

    Specific Activity (EPU /mg protein)

    ≤ 0.8(3 EPU/ml in water)

    Absorbance (277nm)

    ≤0.8 (3EPU/ml in water)

    Purity (HPLC)

    ≥95%

    UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.

    A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit

     

    3.RECOMMEND USAGE

    Effective concentration is equimolar with protease. The recommend binding is pH>6.0 and it will dissociate at pH <3.0.

    Dissolve powder in 0.9%NaCl,then the liquid should be stored at -20℃ or below.

     

    4.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant taprotinin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    5.PRODUCT ADVANTAGE

    Animal origin free: Recombinant aprotinin is no exogenous virus contamination,and any animal origin material is not used in the production process.

    Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

    Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.

    Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    6.PRODUCT USAGE

    Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Recombinant aprotinin is with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes,such as inhibition of serine protease activity in recombinant protein production,cell culture and so on.

     

    7.RELATED PRODUCTS

    Recombinant carboxypeptidase B

    Sequencing grade trypsin

    Recombinant human trypsin

    Recombinant enterokinase

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