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Category:Bio Products > Reagent > Enzyme
Product Name:Recombinant aprotinin solid
CAS No.:
Price(USD):0.00
International
Approvals/Standards:ISO9001:2008
Company:Shanghai Yaxin Biotechnology Co., Ltd.
Factory Location: Shanghai,China
Main Sales Markets: North America,Central/South America,Western Europe,Eastern Europe,Australasia,Asia,Middle East,Africa
Monthly Production Capacity: 5kg
Contract Manufacturing: CRO,CMO
Delivery Lead Time: Within a week
Sample Provided: no
Payment Terms: Payment before Delivery
Recombinant Aprotinin
Catalog Number: RTI16
CAS: 9087-70-1
Synonyms: Trypsin Inhibitor
Source: Recombinant aprotinin is a genetically engineered protein expressed in E.coli
Storage temperature: 2-8℃
Protein Sequence:The amino acid sequence of recombinant aprotinin is identical to bovine lung derived aprotinin
1.DESCRIPTION
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. Effective concentration is equimolar with protease. YaxinBio recombinant aprotinin is a genetically engineered protein expressed in E.coli and purified by multi-column.The amino acid sequence of recombinant trypsin is identical to bovine lung derived aprotinin,with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes.
2.MAIN FEATURES
Source |
Recombinant E. coli |
Appearance |
White or off white, or yellowish powder |
Solubility (5mg/ml in 0.9%NaCl) |
Clear to very slight hazy |
Protein Content [E1% A280 nm = 8.4] |
≥60% |
Specific Activity (EPU /mg protein) |
≤ 0.8(3 EPU/ml in water) |
Absorbance (277nm) |
≤0.8 (3EPU/ml in water) |
Purity (HPLC) |
≥95% |
UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.
A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit
3.RECOMMEND USAGE
Effective concentration is equimolar with protease. The recommend binding is pH>6.0 and it will dissociate at pH <3.0.
Dissolve powder in 0.9%NaCl,then the liquid should be stored at -20℃ or below.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant taprotinin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
Animal origin free: Recombinant aprotinin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Recombinant aprotinin is with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes,such as inhibition of serine protease activity in recombinant protein production,cell culture and so on.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
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