Glucose Oxidase

About The Detailed Introduction Of Glucose Oxidase?

Glucose oxidase powder (GOD) is obtained by deep ventilation fermentation with Penicillium aureus, precipitating it with ethanol and acetone and adsorbing it with kaolin or aluminum hydroxide, and then salting out and refining it with ammonium sulfate. It can also be produced from Penicillium spp. and Aspergillus niger. Almost white to light yellow powder, or yellow to brown liquid. Soluble in water, the aqueous solution is generally light yellow. Almost insoluble in ethanol, chloroform and ether.
It is an important industrial enzyme in the food industry. It is widely used in deoxidation of wine, beer, juice, milk powder and other foods, flour improvement, and prevention of food browning. It is also widely used in rapid food detection and biosensors. GOD is widely distributed in animals, plants and microorganisms. Microorganisms grow and reproduce quickly and have a wide range of sources. They are the main source of GOD production. The main production strains are Aspergillus niger and Penicillium.
Glucose oxidase is an enzyme found in molds such as Penicillium notatum and in honey. It can catalyze the reaction of D-glucose + O2D-gluconic acid (delta-lactone) + H2O2. EC1.1.3.4. The enzyme of Penicillium specificum (P. notatum) has attracted attention due to its obvious antibacterial properties. Therefore, it is also called glucose oxidase (notatin). It is now clear that the antibacterial property is due to the sterilizing properties of H2O2 generated by the reaction. The purified product contains 2 molecules of FAD. As an electron acceptor, in addition to O2, it can also react with 2,6,dichlorophenol and indophenol. This enzyme is specific for glucose.

Enzyme Structure

GOD is a homodimer molecule containing 2 flavin adenine dinucleotide (FAD) binding sites. Each monomer contains 2 completely different regions: one is non-covalently but tightly bound to part of FAD, mainly β-sheet; the other is bound to the substrate β-D-glucose, supported by 4 α-helices and 1 anti- Parallel beta sheets.

Enzymatic Properties

High-purity GOD is a light yellow crystal that is easily soluble in water and can be made into liquid preparations. GOD is completely insoluble in organic solvents such as ether, chloroform, butanol, pyridine, glycerin, and ethylene glycol. The relative molecular mass of GOD is generally around 1.5×105, and each molecule of enzyme contains 2 molecules of FAD. The pH range is 3.5 to 6.5, and the optimal pH is around 5.0. It will be rapidly deactivated at pH >8.0 or pH <3.0 in the absence of a protective agent. The operating temperature range of GOD is generally 30 ~ 60 ℃. Solid enzyme preparations can be stored at 0°C for at least 2 years and at -15°C for 8 years.

What Are The Benefits?

1. GOD consumes oxygen and creates an anaerobic environment:
Thereby inhibiting the growth of harmful bacteria, promoting beneficial bacteria to form dominant flora, improving intestinal flora, and protecting intestinal health;

2. The generated gluconic acid has the effect of acidifying agent:
It can promote the production of more short-chain fatty acids in gastrointestinal chyme and improve intestinal microecology.

3. Produce hydrogen peroxide to sterilize and inhibit bacteria through non-pharmaceutical means:
Hydrogen peroxide has a broad-spectrum bactericidal effect and inhibits the growth and reproduction of pathogenic microorganisms such as Escherichia coli and Salmonella.

4. Protect the integrity of intestinal epithelial cells.
Animals under stress or pathological conditions will produce large amounts of free radicals. When the free radicals exceed the body's own scavenging capacity, they will damage intestinal epithelial cells. Adding GOD can scavenge these free radicals, protect the integrity of intestinal epithelial cells, prevent the invasion of a large number of pathogenic bacteria, improve immunity, and promote animal health and rapid growth.

5. Glucose testing
Widely used in the field of glucose detection. It can convert glucose into gluconic acid by catalyzing the oxidation reaction of glucose, and at the same time generate corresponding electronic or chromatographic signals. This characteristic makes glucose oxidase an important component of blood glucose monitors, biochemical analysis instruments, etc.

6. Food processing
It has a wide range of applications in food processing. It can catalyze the oxidation reaction of glucose and convert glucose into gluconic acid, thereby reducing the glucose content in food and extending the shelf life of food. In addition, glucose oxidase can also catalyze the reaction of glucose and amino acids to produce compounds with special flavors and aromas, which are used in the production of condiments and food additives.

7. Biosensors
It can be combined with other enzymes or substances to construct biosensors. By measuring the current, voltage or light signal changes generated by glucose oxidase catalyzing the glucose oxidation reaction, the glucose content in the organism can be detected and quantitatively analyzed. This kind of biosensor has broad application prospects in fields such as medicine and environmental monitoring.

8. Food brewing
It also plays an important role in the food brewing process. For example, in beer brewing, glucose oxidase can convert glucose in malt into gluconic acid, improving fermentation efficiency and alcohol production. In addition, glucose oxidase can also reduce the residual glucose content in beer and improve the taste and quality of beer.

9.Medical applications
It also has important application value in the medical field. It can be used to treat diabetes by catalyzing the oxidation reaction of glucose, converting glucose in the blood into gluconic acid, thereby reducing blood sugar levels. In addition, glucose oxidase can also be used in the preparation of medical devices such as wound healing and biosensors.

What Are The Application Directions?

Application In Food Industry: Glucose oxidase powder is widely used in the food industry in the manufacturing process of bread, biscuits, candies and other products. It can convert glucose into gluconic acid, thereby playing an antibacterial and antiseptic role and extending the shelf life of food. In addition, glucose oxidase can also be used to detect honey to help determine whether honey is adulterated with glucose.

Application In The Medical Field: It has important application value in the medical field. For example, it can be used in blood glucose monitors to help diabetics monitor blood sugar levels by detecting the amount of glucose in the blood. In addition, glucose oxidase can also be used to prepare some drugs, such as antibiotics and anti-tumor drugs.

Applications In The Field Of Biotechnology: It has a wide range of applications in the field of biotechnology. It can be used in the preparation of biosensors to monitor pollutants in the environment by detecting glucose concentration. Additionally, glucose oxidase can be used to produce hydrogen peroxide, which serves as a clean, highly effective bleach and disinfectant.

Application In Environmental Protection: It can be used to treat organic pollutants in wastewater and exhaust gas. It can oxidize organic matter into carbon dioxide and water, thereby degrading the concentration of organic matter and reducing environmental pollution.

Application In Food Safety: It can be used in food safety testing to help detect whether food contains excessive glucose. This is of great significance for controlling the amount of added sugar in food and ensuring food quality and safety.

Company Introduction And Certificates Obtained

Shaanxi Yuantai Biological Technology Co., Ltd (YTBIO) was established in 2014, mainly supplying Glucose Oxidase powder etc. We have our own factories, R&D and quality inspection teams, and have the conditions for development, production, and quality assurance. At present, we have obtained ISO9001, ISO22000, HALAL, KOSHER, HACCP, FDA,  and other certificates. Therefore, the quality of our products is guaranteed, and it is our original intention to bring high-quality products to customers.

Our Packaging​