Diamond MIX-A mixed-mode resins

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields. However, when doing ion exchange chromatography, it is required that the salt content in the sample should not be too high. The mixed-mode ion exchange resin introduces a benzene ring on the ligand, which makes this type of resin have a certain tolerance to salt, eliminating the desalting step of high salt samples, and expanding the application range of ion exchange.

IEX resin is composed of three parts:

(1)Cross-linked agarose matrix, that has the characteristics of porousness, hydrophobicity and good chemical stability, Diamond base matrix is a high-rigidity agarose base frame, which is made by chemical restructuring and modification of the traditional Bestarose 6FF base frame, with better mechanical properties.

(2)The functional group fixed on the base matrix is a mixed-mode charged group, usually composed of a charged part and a hydrophobic part.

(3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Diamond MIX-A is mixed-mode strong anion exchange resin formed by coupling phenyl glycidyl ether functional groups and 2,3-epoxypropyltrimethylammonium chloride functional groups to high-rigidity agarose microspheres through two steps of crosslinking.