Cytidine 5'-(tetrahydrogen triphos) (CTP-Na2)

Cytidine 5'-(tetrahydrogen triphosphate) disodium salt (CTP-Na₂) is a nucleotide derivative that plays a crucial role in various biological processes. Similar to other nucleotides, CTP-Na₂ is involved in energy transfer, enzymatic reactions, and as a building block for RNA synthesis. Here's an introduction to CTP-Na₂:

Structure: CTP-Na₂ consists of a cytidine molecule, which consists of a pyrimidine base (cytosine) linked to a ribose sugar, with a phosphate group attached to the 5' carbon of the ribose. Additionally, three phosphate groups are attached to the ribose, forming a triphosphate moiety.

Function: CTP-Na₂ serves as an essential component in RNA synthesis and other cellular processes. It functions as a source of energy and as a substrate for enzymes involved in nucleic acid metabolism. CTP is particularly important for the synthesis of cytidine triphosphate (CTP), a building block for RNA molecules.

Applications:

1. RNA synthesis: CTP-Na₂ is incorporated into RNA during transcription, where it acts as a substrate for RNA polymerases. RNA polymerases utilize CTP-Na₂, along with other nucleotides, to extend the growing RNA chain during transcription.

2. Cofactor and substrate: CTP-Na₂ serves as a cofactor for various enzymes involved in nucleotide metabolism. It participates in enzymatic reactions that convert nucleotides to their respective diphosphate or monophosphate forms, contributing to nucleotide pool maintenance and regulation.

3. Research tool: CTP-Na₂ is commonly used in molecular biology research as a substrate, cofactor, or energy source. It is utilized in biochemical assays to study RNA synthesis, RNA-dependent processes, and the activity of enzymes involved in nucleotide metabolism.

CTP-Na₂ is primarily employed in laboratory settings for research purposes rather than for diagnostic or therapeutic applications. Its presence is crucial for the accurate synthesis of RNA molecules and the maintenance of nucleotide pools in cells. By providing the necessary energy and substrates, CTP-Na₂ contributes to the fundamental processes of gene expression and cellular metabolism.