CHO Cell Stable Transfection Serum-free Medium

Chinese hamster ovary (CHO) cells have become the gold standard for biopharmaceutical production and are currently the most widely used mammalian host, especially for the expression of human therapeutic proteins. It has been reported that approximately >70% of recombinant therapeutic proteins are produced in CHO cell systems. CHO cells are capable of more accurate protein glycosylation, assembly and folding like human cells. Therefore, for some proteins whose activity is closely related to post-translational modification, CHO cells become the best production host.The earliest media used for animal-derived cell culture was composed of nutrients such as serum (human or fetal bovine), plasma or tissue extracts, but due to the complex and uncertain components, it caused high variability between batches, and it was easy to increase the risk of contamination. Media without supplementation of serum is a more preferred solution. Serum-free cell culture media are balanced solutions with a chemically defined nutrient composition which is precisely known and defined, ensuring lot-to-lot consistency and eliminating the need to screen new serum lots. For biopharmaceutical manufacturers, who are committed to eliminating all animal-derived components from their manufacturing processes, it is now possible to completely switch to serum-free media solutions when it comes to cell culture media.