What's so magical about DOPE in vaccines?

Pfizer/ Biontech Covid-19 vaccine and Moderna Covid-19 vaccine are familiar with the fact that 1, 2-dioleoyl-SN-glycero-3-phosphoethanolamine is present in both of these formulations. It's called DOPE dioleoyl phosphatidylethanolamine. In this article, we will talk about DOPE in vaccines.

DOPE is a very commonly used helper lipid, which plays a strong synergistic role in the preparation of cationic liposomes. The main principles are as follows:

1. Neutral unstable helper lipids: play the role of stabilizing bilayer and reducing the toxicity of positive components.
2. Interference with lipid membrane and instability of intosomal membrane: it can promote the release of DNA and RNA in cells and assist the cell penetration of cationic liposomes.
3. The morphology of nucleic acid + liposome complex can be determined, but the liposome structure transitions from multiphase Lα phase to hexagonal HII phase, which makes the complex have good fusibility and can improve the transmembrane efficiency.

Therefore, the addition of DOPE can result in better fusion, higher stability and less cytotoxicity of cationic liposome membrane. A large number of studies have shown that lipid polymers alone cannot transfect cells, and the addition of DOPE can solve this problem. For example, the use of DC-CHOL /DOPE can express CAT gene, while the complex DC-CHOL /DOPC has no activity.
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| Chinese name: two oleoyl phosphatidyl ethanolamine
| trade name: DOPE
| chemical name: 1, 2-2 oleoyl - sn - glycerol - 3 - ethanolamine phosphate
| English chemical name: 1, 2 - Dioleoyl - sn - glycero - 3 - phosphoethanolamine
| molecular formula: C41H78NO8P
| manufacturers: YiWeiTa (Shanghai) pharmaceutical technology co., LTD
| CAS number: 4004-5-1
| DMF number: 028884
| EINECS no. : 200-663-8
| levels: medicinal reagent level
| executive standard: enterprise standard
| purpose: liposomes
| solubility: insoluble in water, soluble in chloroform, methanol, ethanol
| molecular weight: 744.03
| preservation conditions: - 20 ℃
| note: avoid contact with strong acid, strong alkali, strong oxidizing substances

For phospholipids medical accessories such as DOPE have a demand for small partners welcome to inquire: 400-6262 623!