What is the role of DOTMA dioleoylpropyl trimethylammonium chloride in cationic liposomes during RNA

DOTMA dioleylpropyl trimethylammonium chloride plays an important role in cationic liposomes during RNA drug development. In recent years, research on non-viral vectors for gene therapy has made some achievements, among which the most important is the use of cationic liposomes. In 1987, Felgner et al. Ci prepared N-[1-(2,3) -diolenoxy] propyl -N,N, n-trimethylamine chloride (DOTMA) and dioleoylphosphatidyloethanolamine (DOPE) 10mg each into a small single-chamber Lipofectin (transfection reagent, trade name Lipofectin, transformed lipid). DOTMA is also used as a component of liposomes and granules in the development of siRNA drugs.

With the understanding of the pathogenesis of diseases on the cellular level, gene therapy has become a hot topic in the research of scientists. Finding safe and effective gene transfer vectors has become more and more important. At present, viral vectors are mainly used, such as recombinant adenovirus vectors, which have certain advantages in gene transfer due to their high transfection efficiency and targeting effect on most cells. However, because it causes some immune responses in the body, and contains transcribable viral genes, gene recombination or complementing may occur in the body, causing further harm to the human body.

Liposomes or lipid particles are composed of lipid molecules, such as phospholipids, cholesterol, etc., which are encapsulated by membrane materials. According to electric charge, liposomes can be divided into neutral liposomes, negative liposomes and positive liposomes. Liposomes have been widely used in scientific research and drug development. Mixing positive lipid molecules with neutral lipid molecules can be used to wrap nucleic acid molecules, such as DNA, RNA, small interference nucleic acid, MiRNA, etc., for gene therapy, gene inhibition, this technology is widely used in research and development of new drugs. Liposomes, or lipid molecules, have also been used to wrap some drugs to improve their targeting, stability, effectiveness, and side effects.

Liposomes can not be widely used in research, its biggest clinical disadvantage is: the toxicity and effectiveness of liposomes restrict its application, especially in drug development. The method of improving liposomes or lipid particles is mainly carried out from several aspects: for example, composition. Liposome, lipid particles usually have a mixture of several components, mainly phospholipid, cholesterol, in order to enhance the stability of liposome, particles and half-life in the body, liposome, particle surface through a lipid PEG molecule, protect lipid nanoparticles, should not be aggregated and phagocytosis by leukocytes. Among all the factors affecting the function and application of liposomes, the components of liposomes play a significant role in zhu. At present, cationic liposomes dominate the market in nucleic acid applications (research and drug development). However, the toxicity and effectiveness of cationic liposomes are still the key obstacles to the application of cationic liposomes.

In addition to DOTMA, dLIN-MC3-DMA, DMG-PEG2000, DOTAP, DC-CHOL, DODMA, DOPE, DOP-DEDA, etc., injection grade excipients, Apply to RNA liposomes.

The following AVT xiaobian will introduce the basic information of cationic lipid materials DOTMA:

Product name: DOTMA

Common name: DOTMA

Chinese name: trimethylammonium dioleoylpropyl chloride

Chemical Name: 1, 2-dioctotenoxy-3-methylammonium propane (chloride salt)

Molecular formula: C42H84ClNO2

Manufacturer: Avito (Shanghai) Pharmaceutical Technology Co., LTD

CAS no. : 104872-42-6

Purpose: cationic liposome

Formula:

What is the role of DOTMA dioleoylpropyl trimethylammonium chloride in cationic liposomes during RNA drug development

Properties: white solid

Purity: above 96%

Molecular weight: 670.575

Melting point: 35 ~ 38 ℃

Storage conditions: -20℃

The application of DOTMA dioleoylpropyl trimethylammonium chloride in cationic liposomes is introduced here.

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