The basic information and advantages and disadvantages of commonly used cationic liposome DOTMA

Dotma was born in 1987. Felgner et al. synthesized it and applied it to the delivery of DNA. Studies have shown that the single-compartment liposomes prepared by DOTMA can contain high amounts of DNA and RNA segments, and can be well absorbed by cells to express target genes, and the transfection efficiency at the cellular level is 10 times higher than that of DOTAP. In this video, AVT will introduce you to the basic information, advantages and disadvantages of DOTMA.


Compared with DOTAP, DC-Chol, DoDMA, etc., DOTMA has higher transfection efficiency and less cytotoxicity. Therefore, more and more studies have been conducted on the development of medicinal cationic liposomes.

In terms of molecular structure, DOTMA has the characteristic of "bigger tail fan, smaller head", and is better than DC-CHOL and DOEPC in terms of liposome micromorphology, in vivo stability and interaction with cell membrane.

DOTMA advantages:

· High nucleic acid encapsulation efficiency, RNA encapsulation rate in RNA/DOTMA lipid complex can reach 100%;

· High gene transfection efficiency, more than 10 times higher than Dotapchu formula on some cells;

· Low cytotoxicity and good safety;

· Good for GMP mass production.

DOTMA faults:

· High price;

· The transfection efficiency is lower than that of ionizable cationic lipids such as DLIN-MC3-DMA;

· It is still in the laboratory research stage

Common name: DOTMA

English name: trimethylammonium dioleopropyl chloride

Chemical name: 1, 2-didecaenooxy-3-methylammonium propane (chlorine salt)

CAS no. : 104872-42-6

Properties: white solid

Molecular Weight: 670.575

Melting point: 35 ~ 38 ℃

Preservation condition: -20℃

Precautions: avoid contact with strong acid, strong alkali and strong oxidizing substances

Application: cationic liposomes

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