DMF 032797 DPPG phospholipid - microbubble contrast agent

AVT supplies you with high quality DPPG phospholipid, which is often used as a microbubble contrast agent, so you can often see it. Deep cultivation of pharmaceutical accessories industry for more than ten years, xiaobian to introduce you to AVT this has been registered in the FDA DMF 032797 DPPG phospholipid is a kind of phospholipid? How does it work as a microbubble contrast agent?

What kind of phospholipid is DPPG?

DPPG Phospholipid -- Microbubble Contrast agent -- Avitol (Shanghai) Pharmaceutical Technology Co., LTD

English name: DPPG-NA

Chemical Name: 1, 2-dipalmitoyl-Sn-glycerol -3-[phosphoric acid-RAC -(1-glycerol), sodium salt]

CAS no. : 67232-81-9

Registration Number: F20180000838

DMF number: 32797

Molecular weight: 745

Molecular formula: C38H74O10PNa

Grade: Medicinal injection grade

Application: microbubble contrast agent, liposome membrane material, emulsifier, etc

Product properties: white powder.

Solubility: soluble in chloroform/methanol =2/1 (V/V)

Storage conditions: below -20℃, shaded and sealed

Application of DPPG phospholipids in liposomes

DPPG is suitable for the preparation of anionic liposomes, which is a common choice of negative charge membrane material and plays a key role in improving the stability of liposomes. Liposome drug development and the prescription of marketed products are mostly used, and some liposome projects are also used in the clinical stage, such as cisplatin Lipoplatin.

How does DPPG phospholipid function as a microbubble contrast agent? Here are a few chestnuts.

1. Preliminary Study on Preparation of Ultrasonic Contrast Agent targeting Liposome Microbubbles with Long Cycle

Authors: Xie Mingxing, ZHANG Li, LIU Yingying, Feng Min, WANG Xinfang, LU Qing

To investigate the feasibility of using biotin-avidin-biotin (BSB) bridging technology to prepare long cycle targeted liposome microbubble (LCLM) ultrasound contrast agent carrying rabbit anti-human fibrinogen. The prescribed amount of DPPC,DPPE,DPPG,DSPE-PEG2000, DSPE-PEG2000-biotin, cholesterol and other lipid materials were weighed to prepare targeted ultrasound contrast agent.

2. Preparation of Cationic Liposome Microbubble Ultrasound Contrast Agent and Preliminary Study on Gene Transfection in Vitro

Author: Li Diancheng

A cationic liposome microbubble ultrasound contrast agent that can be observed in real time by conventional ultrasound was prepared to provide a carrier that can be observed in real time, thus providing certain technical support for gene carrying microbubble combined with ultrasound targeted therapy.

Three lipids, DPPC,1, 2-diolenoxy-3-trimethylaminopropane (DOTAP) and distearoylphosphatidylethanolamine-polyethylene glycol 2000(DSPE-PEG2000), were mixed in a certain proportion to prepare liposome microbubble ultrasonic contrast agent by thin film dispersion method. The morphology of microbubbles was observed under a light microscope, the particle size distribution of microbubbles was detected by a laser particle size analyzer, and the development effect of microbubbles in vivo and in vitro was observed by a medical ultrasonic diagnostic instrument. In vivo development effect was compared with commercial ultrasound contrast agent (sonovine) to observe the difference. The ability of liposome microbubbles to carry PGL3-control gene was detected by DNA gel electrophoresis, and the transfection effect of the microbubbles in vitro was analyzed.

Results: The average particle size of liposome microbubbles prepared by mixing DPPC,DOTAP and DSPE-PEG2000 was 6.969μm. In vitro and in vivo development effect of ultrasound contrast agent was good, and there was no statistical difference in the development duration of large arteries in vivo compared with sonovi (P >0.05). DNA gel electrophoresis experiments showed that the self-made cationic liposome microbubble ultrasound contrast agent had a better ability to carry PGL3-control gene, and the carrying ability was significantly better than that of Sonovi. Meanwhile, sonovi was also found to have a very weak ability to carry PGL3-control gene. In vitro cell experiments confirmed that the self-made liposome microbubbles significantly improved the transfection efficiency of PGL3-control in COS-7 cells (P <0.01).

DPPG is used as injection grade dipalmitoylphosphatidylglycerol sodium, a fully synthetic phospholipid, which needs to be shaded, sealed and refrigerated to avoid co-storage with strong oxidizing substances. Phospholipids can hydrolyze in strong acid and alkali environment, so in addition to strong oxidizing substances, contact with strong acid or alkali media should also be avoided.

The microbubble contrast agent DPPG phospholipid interested partners welcome to inquire: 400-6262 623!