Cationic Lipid DOTMA

What is DOTMA? In recent years, the concept of gene therapy, antibody therapy, immunotherapy, etc. has been overwhelming, and the hot gene delivery has become the focus of research. The AVT editor who focuses on the development of excipients thought this was an emerging field, but after a simple investigation, it was discovered that scientific researchers had successfully used cationic lipid excipients to prepare liposomes for gene transfection as early as 30 years ago.The protagonist DOTMA to be introduced in this article has such a background.

DOTMA was synthesized and applied to DNA delivery by Felgner et al. in 1987. It was found that the single chamber liposome prepared by DOTMA could efficiently package DNA and RNA fragments with 100% encapsulation rate, and could be well taken up by cells to express target genes, with 10 times higher transfection efficiency at the cellular level than DOTAP. However, DOTMA is not well known by domestic cationic liposome researchers, and is more commonly used in DOTAP and DC-CHOL.
DOTMA, known as dioleoyl propyl trimethyl ammonium chloride (DOPTMA) in Chinese, has a better transfection effect than the long chain (C16 or C18) liposomes composed of two short chains of fatty acids (C14), with the following structural formula.

The DODMA that can be easily confused with it is 1,2-dioleoyloxy-3-dimethylaminopropane. The two ammonium ion valences are different, the charge density is different, and the usage and effect are naturally different.

The effect of RNA drugs is closely related to the selected cationic lipid materials. When you encounter problems in the experiment, you may wish to try several cationic lipid materials. What's the basic information of DOTMA?
1. Common name: DOTMA
2. Product name: DOTMA
3. Chinese name: Dioleyl propyl trimethylammonium chloride
4. Chemical name: 1,2-Dioctadecenyloxy-3-methylammonium propane (chloride salt)
5. CAS number: 104872-42-6
6. Manufacturer: AVT (Shanghai) Pharmaceutical Tech Co,. Ltd.
7. Molecular formula: C42H84ClNO2
8. Application: cationic liposome
9. Hygroscopicity: None
10. Properties: white solid
11. Purity: above 96%
12. Molecular weight: 670.575
13. Melting point: 35~38℃
14. Photosensitivity: Yes
15. Storage conditions: -20℃
AVT brings you a variety of cationic lipid materials that can meet various needs in the experiment. Interested friends are welcome to call 400-6262 623!